ARG65479
anti-MHC Class II antibody [M5/114] (azide free)
anti-MHC Class II antibody [M5/114] (azide free) for Flow cytometry,Functional study,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse
Immune System antibody
概述
| 产品描述 | Azide free Rat Monoclonal antibody [M5/114] recognizes MHC Class II |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, FuncSt, IHC-Fr, IHC-P, IP, WB |
| 特异性 | The clone M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | M5/114 |
| 同位型 | IgG2b |
| 靶点名称 | MHC Class II |
| 抗原物种 | Mouse |
| 抗原 | Activated C57BL/6 mouse spleen cells. |
| 偶联标记 | Un-conjugated |
| 別名 | HLA-DRB; HLA class II histocompatibility antigen, DRB1-3 chain; SS1; MHC class II antigen DRB1*3; HLA-DR1B; DRw10; Clone P2-beta-3; DRB1 |
应用说明
| 应用建议 |
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| 应用说明 | Functional Application: Blocking of T cell proliferative responses * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 纯化说明 | 0.2 µm filter sterilized. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | H2-D1 |
|---|---|
| 全名 | histocompatibility 2, D region locus 1 |
| 背景介绍 | MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long. |
| 生物功能 | Involved in the presentation of foreign antigens to the immune system. [UniProt] |
| 研究领域 | Immune System antibody |
| 预测分子量 | 30 kDa |
| 翻译后修饰 | Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to sorting into the endosome system and down-regulation of MHC class II. |
检测图片 (1) Click the Picture to Zoom In
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ARG65479 anti-MHC Class II antibody [M5/114] (azide free) FACS image
Flow Cytometry: Murine splenocytes stained with ARG65479 anti-MHC Class II antibody [M5/114] (azide free), followed by APC-conjugated Goat anti-Rat antibody.
