ARG23007
anti-MICA + MICB antibody [6D4] (low endotoxin)
anti-MICA + MICB antibody [6D4] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation and Human
概述
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [6D4] recognizes MICA + MICB Mouse anti Human MICA/Micb antibody, clone 6D4 recognizes nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB). MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells (Jinushi et al. 2003). |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IHC-Fr, IP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6D4 |
| 同位型 | IgG2a |
| 靶点名称 | MICA + MICB |
| 抗原物种 | Human |
| 抗原 | MICA transfected C1R cells. |
| 偶联标记 | Un-conjugated |
| 別名 | MHC class I polypeptide-related sequence B; MIC-B; PERB11.2 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Membrane permeabilisation is required for this application. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 纯化说明 | Low endotoxin |
| 缓冲液 | PBS |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q29980 Human MHC class I polypeptide-related sequence B |
|---|---|
| 基因名称 | MICB |
| 全名 | MHC class I polypeptide-related sequence B |
| 背景介绍 | This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt] |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | Proteolytically cleaved and released from the cell surface of tumor cells. |
