ARG41492
anti-MLKL phospho (Ser345) antibody
anti-MLKL phospho (Ser345) antibody for IHC-Formalin-fixed paraffin-embedded sections and Mouse
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody recognizes MLKL phospho (Ser345) |
|---|---|
| 反应物种 | Ms |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 同位型 | IgG |
| 靶点名称 | MLKL |
| 抗原物种 | Mouse |
| 抗原 | Phosphospecific peptide around Ser345 of Mouse MLKL. |
| 偶联标记 | Un-conjugated |
| 別名 | Mixed lineage kinase domain-like protein; hMLKL |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | IHC-P: Mouse colon |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | TBS (pH 7.4), 0.05% Sodium azide, 40% Glycerol and 1% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 40% Glycerol and 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9D2Y4 Mouse Mixed lineage kinase domain-like protein |
|---|---|
| 基因名称 | MLKL |
| 全名 | mixed lineage kinase domain-like |
| 背景介绍 | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction. [UniProt] |
| 产品亮点 | Related products: MLKL antibodies; MLKL Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: RIP1 activation and pathogenesis of NASH Ripoptosome & Necrosome antibody panels are launched |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG41492 anti-MLKL phospho (Ser345) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse colon tissue stained with ARG41492 anti-MLKL phospho (Ser345) antibody. Counter stained with hematoxylin.
