ARG66709
anti-MMP1 antibody
anti-MMP1 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MMP1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MMP1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide within the C-terminal region of Human MMP1. |
| 偶联标记 | Un-conjugated |
| 別名 | MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 54 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP1 |
| 全名 | matrix metallopeptidase 1 |
| 背景介绍 | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009] |
| 生物功能 | Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Tyrosine phosphorylated in platelets by PKDCC/VLK. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66709 anti-MMP1 antibody ICC/IF image
Immunofluorescence: Formalin-fixed MCF7 cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66709 anti-MMP1 antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. DAPI (blue) for nuclear staining.
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ARG66709 anti-MMP1 antibody WB image
Western blot: HeLa, MCF7, MDA-MB-435, Raw264.7, PC12 and NIH/3T3 whole cell lysates stained with ARG66709 anti-MMP1 antibody.
