ARG20154
anti-MMP13 antibody
anti-MMP13 antibody for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Horse
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
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概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MMP13 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov, Hrs |
| 应用 | IHC-Fr, IHC-P, IP, WB |
| 特异性 | The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13. Reactivity to other species has not been tested. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MMP13 |
| 抗原物种 | Rat |
| 抗原 | Synthetic peptide surrounding amino acid 454 of rat MMP-13 |
| 偶联标记 | Un-conjugated |
| 別名 | MMP13; Matrix Metallopeptidase 13; Collagenase 3; CLG3; Matrix Metalloproteinase 13 (Collagenase 3); MMP-13; Matrix Metalloproteinase-13; EC 3.4.24.35; EC 3.4.24.22; EC 3.4.24.24; EC 3.4.24.65; EC 3.4.24.; EC 3.4.24.7; EC 3.4.24; MANDP1; MDST |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified Antibody |
| 缓冲液 | PBS, 30% Glycerol, 0.5% BSA and 0.01% Thimerosal |
| 抗菌剂 | 0.01% Thimerosal |
| 稳定剂 | 30% Glycerol, 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP13 |
| 全名 | matrix metallopeptidase 13 |
| 背景介绍 | This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016] |
| 生物功能 | Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. [UniProt] |
| 细胞定位 | Extracellular matrix, Secreted. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG20154 anti-MMP13 antibody WB image
Western blot: Jurkat cell lysate stained with ARG20154 anti-MMP13 antibody.
The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13.
客户反馈
Average
anti-MMP13 antibody
Application：WB
Sample：A: MDA-MB-231, B: MCF-7
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
