ARG40476
anti-MMP16 antibody
anti-MMP16 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MMP16 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MMP16 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to Y120-I296 of Human MMP16. |
| 偶联标记 | Un-conjugated |
| 別名 | MT-MMP 3; MT-MMP2; MT3-MMP; MTMMP3; MT-MMP3; C8orf57; EC 3.4.24.-; MMP-X2; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP-16; MT3MMP; Matrix metalloproteinase-16 |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP16 |
| 全名 | matrix metallopeptidase 16 (membrane-inserted) |
| 背景介绍 | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010] |
| 生物功能 | Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. [UniProt] |
| 细胞定位 | Isoform Long: Cell membrane; Single-pass type I membrane protein; Extracellular side. Note=Localized at the cell surface of melanoma cells. Isoform Short: Secreted, extracellular space, extracellular matrix. Cell surface. Note=Localized at the cell surface of melanoma cells. [UniProt] |
| 预测分子量 | 70 kDa |
| 翻译后修饰 | The precursor is cleaved by a furin endopeptidase. [UniProt] |
检测图片 (7) Click the Picture to Zoom In
-
ARG40476 anti-MMP16 antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG40476 anti-MMP16 antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
-
ARG40476 anti-MMP16 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40476 anti-MMP16 antibody at 1 µg/ml, overnight at 4°C.
-
ARG40476 anti-MMP16 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Mouse intestine and SMMC-7721 whole cell lysates stained with ARG40476 anti-MMP16 antibody at 0.5 µg/ml, overnight at 4°C.
-
ARG40476 anti-MMP16 antibody FACS image
Flow Cytometry: Caco-2 cells were blocked with 10% normal goat serum and then stained with ARG40476 anti-MMP16 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
-
ARG40476 anti-MMP16 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40476 anti-MMP16 antibody at 1 µg/ml, overnight at 4°C.
-
ARG40476 anti-MMP16 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40476 anti-MMP16 antibody at 1 µg/ml, overnight at 4°C.
-
ARG40476 anti-MMP16 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40476 anti-MMP16 antibody at 1 µg/ml, overnight at 4°C.
