ARG40477

anti-MMP16 antibody

anti-MMP16 antibody for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes MMP16
反应物种 Hu, Rat
预测物种 Ms, Hm
应用 IHC-Fr, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 MMP16
抗原物种 Human
抗原 Synthetic peptide corresponding to aa. 582-598 of Human MMP16. (YTVFQFKRKGTPRHILY)
偶联标记 Un-conjugated
別名 MT-MMP 3; MT-MMP2; MT3-MMP; MTMMP3; MT-MMP3; C8orf57; EC 3.4.24.-; MMP-X2; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP-16; MT3MMP; Matrix metalloproteinase-16

应用说明

应用建议
应用 推荐稀释比
IHC-Fr1:200 - 1:1000
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
抗菌剂 0.05% Thimerosal and 0.05% Sodium azide
稳定剂 5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4325 Human MMP16

GeneID: 65205 Rat MMP16

Swiss-port # O35548 Rat Matrix metalloproteinase-16

Swiss-port # P51512 Human Matrix metalloproteinase-16

基因名称 MMP16
全名 matrix metallopeptidase 16 (membrane-inserted)
背景介绍 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010]
生物功能 Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. [UniProt]
细胞定位 Isoform Long: Cell membrane; Single-pass type I membrane protein; Extracellular side. Note=Localized at the cell surface of melanoma cells. Isoform Short: Secreted, extracellular space, extracellular matrix. Cell surface. Note=Localized at the cell surface of melanoma cells. [UniProt]
预测分子量 70 kDa
翻译后修饰 The precursor is cleaved by a furin endopeptidase. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG40477 anti-MMP16 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40477 anti-MMP16 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG40477 anti-MMP16 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40477 anti-MMP16 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG40477 anti-MMP16 antibody WB image

    Western blot: 50 µg of samples under reducing conditions. HeLa and COLO320 whole cell lysates stained with ARG40477 anti-MMP16 antibody at 0.5 µg/ml, overnight at 4°C.

  • ARG40477 anti-MMP16 antibody WB image

    Western blot: 30 µg of sample under reducing conditions. Human heart lysate stained with ARG40477 anti-MMP16 antibody at 0.5 µg/ml dilution, overnight at 4°C.

  • ARG40477 anti-MMP16 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40477 anti-MMP16 antibody at 1 μg/ml dilution, overnight at 4°C.