概述

产品描述	Rabbit Polyclonal antibody recognizes MMP2
反应物种	Hu
应用	ELISA, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	MMP2
抗原物种	Human
抗原	Human MMP2 recombinant protein
表达系统	E.coli
偶联标记	Un-conjugated
Protein Full name	72 kDa type IV collagenase
別名	MMP2; Matrix Metallopeptidase 2; TBE-1; CLG4A; 72 KDa Type IV Collagenase; Matrix Metalloproteinase-2; EC 3.4.24.24; MMP-2; CLG4; Matrix Metalloproteinase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metallopeptidase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase) ; Matrix Metalloproteinase-II; Collagenase Type IV-A; Neutrophil Gelatinase; 72 KDa Gelatinase; Gelatinase A; EC 3.4.24; MMP-II; MONA

应用说明

应用建议	应用 推荐稀释比 ELISA 0.1-0.5 µg/ml WB 0.25-0.5 µg/ml
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purified with Immunogen.
缓冲液	0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
稳定剂	4% Trehalose
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4313 Human MMP2 Swiss-port # P08253 Human 72 kDa type IV collagenase
基因名称	MMP2
全名	Matrix Metallopeptidase 2
背景介绍	This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
生物功能	Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.
细胞定位	Cytoplasm, Extracellular matrix, Membrane, Mitochondrion, Nucleus, Secreted
预测分子量	74 kDa
翻译后修饰	Autocatalytic cleavage, Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

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