ARG66423
anti-MMP7 (total) antibody [SQab18125]
anti-MMP7 (total) antibody [SQab18125] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [SQab18125] recognizes MMP7 (total) |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SQab18125 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | MMP7 (total) |
| 抗原物种 | Human |
| 抗原 | Recombinant Human MMP7 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | MMP-7; Uterine metalloproteinase; Matrix metalloproteinase-7; EC 3.4.24.23; Matrilysin; Matrin; Pump-1 protease; PUMP-1; MPSL1 |
应用说明
| 应用建议 |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 纯化 | Affinity purification with immunogen. |
|---|---|
| 缓冲液 | PBS (pH 7.4) and 0.01% Thimerosal. |
| 抗菌剂 | 0.01% Thimerosal |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP7 |
| 全名 | matrix metallopeptidase 7 |
| 背景介绍 | MMP7 is a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers. [provided by RefSeq, Jan 2016] |
| 生物功能 | MMP7 degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 预测分子量 | 30 kDa |
检测图片 (4) Click the Picture to Zoom In
-
ARG66423 anti-MMP7 (total) antibody [SQab18125] ICC/IF image
Immunofluorescence: A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66423 anti-MMP7 (total) antibody [SQab18125] (green) at 1:200 dilution and cell nuclei were stained with Hoechst 33342 (blue).
-
ARG66423 anti-MMP7 (total) antibody [SQab18125] FACS image
Flow Cytometry: A549 cells were stained with ARG66423 anti-MMP7 (total) antibody [SQab18125] at 2 µg/ml (red) and without antibody control (black).
-
ARG66423 anti-MMP7 (total) antibody [SQab18125] WB image
Western blot: 50 ng of Recombinant Pro-MMP7, 50 ng of Recombinant MMP7 (total), 30 µg of 293T, 30 µg of LPS treated 293T, 30 µg of MMP7 transfected 293T and 30 µg of LPS treated MMP7 transfected 293T cell lysates stained with ARG66423 anti-MMP7 (total) antibody [SQab18125] at 1:5000 dilution. The expression of Actin was as the internal control.
-
ARG66423 anti-MMP7 (total) antibody [SQab18125] ELISA image
ELISA: Titration curve of ARG66423 anti-MMP7 (total) antibody [SQab18125]. Red: MMP7 (active); Green: MMP7 (proenzyme); Black: BSA (negative control).
