ARG43747
anti-MPO / Myeloperoxidase antibody
anti-MPO / Myeloperoxidase antibody for Flow cytometry,Western blot and Human
Inflammatory Cell Marker antibody; Neurophil Marker antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MPO / Myeloperoxidase |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MPO / Myeloperoxidase |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to S406-S745 of Human Myeloperoxidase / MPO. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Myeloperoxidase |
| 別名 | MPO; Myeloperoxidase; EC 1.11.2.2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HL-60 | ||||||
| 实际分子量 | 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MPO |
| 全名 | myeloperoxidase |
| 背景介绍 | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| 生物功能 | Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt] |
| 细胞定位 | Lysosome. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30325 Inflammatory Cell Antibody Panel Related products: MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Inflammatory Cell Marker antibody; Neurophil Marker antibody |
| 预测分子量 | 60 kDa, 84 kDa, 89 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG43747 anti-MPO / Myeloperoxidase antibody WB image
Western blot: 30 µg of HL-60 lysate under reducing conditions stained with ARG43747 anti-MPO / Myeloperoxidase antibody at 0.5 µg/ml, overnight at 4°C.
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ARG43747 anti-MPO / Myeloperoxidase antibody FACS image
Flow Cytometry: Human HL-60 cells were blocked with 10% normal goat serum and then stained with ARG43747 anti-MPO / Myeloperoxidase antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
