ARG43748
anti-MPO / Myeloperoxidase antibody [MPO/33R]
anti-MPO / Myeloperoxidase antibody [MPO/33R] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Inflammatory Cell Marker antibody; Neurophil Marker antibody
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [MPO/33R] recognizes MPO / Myeloperoxidase |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | MPO/33R |
| 同位型 | IgG |
| 靶点名称 | MPO / Myeloperoxidase |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 150-250 of Human MPO / Myeloperoxidase. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Myeloperoxidase |
| 別名 | MPO; Myeloperoxidase; EC 1.11.2.2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human spleen | ||||||
| 实际分子量 | 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A/G affinity. |
| 缓冲液 | PBS, 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MPO |
| 全名 | myeloperoxidase |
| 背景介绍 | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| 生物功能 | Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt] |
| 细胞定位 | Lysosome. [UniProt] |
| 产品亮点 | Related products: MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Inflammatory Cell Marker antibody; Neurophil Marker antibody |
| 预测分子量 | 60 kDa, 84 kDa, 89 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG43748 anti-MPO / Myeloperoxidase antibody [MPO/33R] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human spleen tissue. Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 20 min, followed by cooling at RT. The tissue section was stained with ARG43748 anti-MPO / Myeloperoxidase antibody [MPO/33R].
Negative control inset: PBS instead of primary antibody to control for secondary binding.
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ARG43748 anti-MPO / Myeloperoxidase antibody [MPO/33R] WB image
Western blot: Human spleen lysate stained with ARG43748 anti-MPO / Myeloperoxidase antibody [MPO/33R].
