ARG43052
anti-MUC13 antibody
anti-MUC13 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MUC13 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MUC13 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to E226-S312 of Human MUC13. |
| 偶联标记 | Un-conjugated |
| 別名 | MUC-13; DRCC1; Mucin-13; Down-regulated in colon cancer 1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MUC13 |
| 全名 | mucin 13, cell surface associated |
| 背景介绍 | Epithelial mucins, such as MUC13, are a family of secreted and cell surface glycoproteins expressed by ductal and glandular epithelial tissues (Williams et al., 2001 [PubMed 11278439]).[supplied by OMIM, Jul 2008] |
| 生物功能 | Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Apical cell membrane. Secreted. Note=Also exists as a soluble form. [UniProt] |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homooligomerization into dimers is dependent on intrachain disulfide bonds. Highly N-glycosylated. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
-
ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human appendicitis tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43052 anti-MUC13 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Human placenta, Caco-2, PC-3, U2OS, U-87MG, HepG2 and HeLa whole cell lysates stained with ARG43052 anti-MUC13 antibody at 0.5 µg/ml dilution, overnight at 4°C.
-
ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
