ARG66202
anti-MUC16 / CA125 antibody [SQab1724]
anti-MUC16 / CA125 antibody [SQab1724] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1724] recognizes MUC16 / CA125 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1724 |
| 同位型 | IgG |
| 靶点名称 | MUC16 / CA125 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 12200‐12300 of MUC16 / CA125. |
| 偶联标记 | Un-conjugated |
| 別名 | Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MUC16 |
| 全名 | mucin 16, cell surface associated |
| 生物功能 | Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt] |
| 产品亮点 | Related products: MUC16 antibodies; MUC16 ELISA Kits; Related news: Cancer Pathology Markers (SQ clones) |
| 预测分子量 | 1519 kDa |
| 翻译后修饰 | Heavily O-glycosylated; expresses both type 1 and type 2 core glycans. Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. |
检测图片 (4) Click the Picture to Zoom In
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ARG66202 anti-MUC16 / CA125 antibody [SQab1724] WB image
Western blot: 30 µg of HeLa and A431 cell lysates stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] at 1:500 dilution.
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ARG66202 anti-MUC16 / CA125 antibody [SQab1724] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] (red) at 1:800 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66202 anti-MUC16 / CA125 antibody [SQab1724] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human ovarian cancer tissue stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] at 1:400 dilution.
Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
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ARG66202 anti-MUC16 / CA125 antibody [SQab1724] IHC-P image
Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human ovarian cancer tissue stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] at 1:400 dilution.
Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
客户反馈
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anti-MUC16 / CA125 antibody [SQab1724]
Application：WB
Sample：HeLa and A431
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
