ARG10156

anti-MUC16 / CA125 antibody [X75]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

50 μg

279

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [X75] recognizes MUC16 / CA125
反应物种	Hu
应用	ELISA, FACS, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	X75
同位型	IgG1
靶点名称	MUC16 / CA125
抗原物种	Human
抗原	MUC16 / CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).
抗原表位	Epitope specificity group B (ISOBM classification) similar to M-11.
偶联标记	Un-conjugated
別名	Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125

应用说明

应用建议

应用	推荐稀释比
ELISA	Assay-dependent
FACS	0.1 µg/10^6 cells
ICC/IF	Assay-dependent
IHC-P	1 - 5 µg/ml
WB	1 - 5 µg/ml

应用说明

Sandwich ELISA (Capture antibody - Detection antibody):
ARG10156 - ARG10351
ARG10351 - ARG10156
ARG10534 - ARG10156

IHC-P: Antigen Retrieval: Heat mediation was performed.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Ion exchange chromatography.
缓冲液	10 mM Tris (pH 7.5), 0.15 M NaCl and 0.05% Sodium azide.
抗菌剂	0.05% Sodium azide
浓度	1.0-2.0 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 94025 Human MUC16 Swiss-port # Q8WXI7 Human Mucin-16
基因名称	MUC16
全名	mucin 16, cell surface associated
生物功能	Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt]
研究领域	Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
预测分子量	1519 kDa
翻译后修饰	Heavily O-glycosylated; expresses both type 1 and type 2 core glycans. Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

检测图片 (3) Click the Picture to Zoom In

ARG10156 anti-MUC16 / CA125 antibody [X75] IHC-P image

Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed. The tissue section was stained with ARG10156 anti-MUC16 / CA125 antibody [X75] at 1:200 dilution.
ARG10156 anti-MUC16 / CA125 antibody [X75] standard curve image

ELISA: Analysis of Human CA125 expression using ARG10156 anti-MUC16 / CA125 antibody [X75] as a capture antibody and biotinylated ARG10351 anti-MUC16 / CA125 antibody [X325] as a detection antibody with purified Human CA125 as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Plasma (green) sample at 1:2 is shown.
ARG10156 anti-MUC16 / CA125 antibody [X75] standard curve image

ELISA: Analysis of Human CA125 expression using ARG10351 anti-MUC16 / CA125 antibody [X325] as a capture antibody and biotinylated ARG10156 anti-MUC16 / CA125 antibody [X75] as a detection antibody with purified Human CA125 as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Plasma (green) sample at 1:2 is shown.