ARG66752
anti-MYH11 / SMMHC antibody [SQab20185]
anti-MYH11 / SMMHC antibody [SQab20185] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab20185] recognizes MYH11 / SMMHC |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab20185 |
| 同位型 | IgG |
| 靶点名称 | MYH11 / SMMHC |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 1-100 of Human MYH11 / SMMHC. |
| 偶联标记 | Un-conjugated |
| 別名 | Myosin-11; Myosin heavy chain, smooth muscle isoform; AAT4; SMMHC; FAA4; SMHC; Myosin heavy chain 11 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Liomyoma tissue. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MYH11 |
| 全名 | myosin, heavy chain 11, smooth muscle |
| 背景介绍 | The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Muscle contraction. [UniProt] |
| 细胞定位 | Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils. [UniProt] |
| 预测分子量 | 227 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG66752 anti-MYH11 / SMMHC antibody [SQab20185] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human liomyoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66752 anti-MYH11 / SMMHC antibody [SQab20185] at 18°C - 25°C for 30 minutes.
