ARG66249
anti-Melanoma gp100 antibody [SQab1738]
anti-Melanoma gp100 antibody [SQab1738] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1738] recognizes Melanoma gp100 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1738 |
| 同位型 | IgG |
| 靶点名称 | Melanoma gp100 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human Melanoma gp100. |
| 偶联标记 | Un-conjugated |
| 別名 | Premelanosome protein; SILV; ME20; Melanocyte protein Pmel 17; ME20-M; Secreted melanoma-associated ME20 antigen; 95 kDa melanocyte-specific secreted glycoprotein; Silver locus protein homolog; ME20S; D12S53E; SIL; P1; Melanocyte protein PMEL; PMEL17; ME20-S; Melanoma-associated ME20 antigen; gp100; ME20M; P100; SI; P26; Melanocytes lineage-specific antigen GP100 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PMEL |
| 全名 | premelanosome protein |
| 背景介绍 | This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011] |
| 生物功能 | Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. [UniProt] |
| 产品亮点 | Related products: Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| 预测分子量 | 70 kDa |
| 翻译后修饰 | A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides. N-glycosylated. O-glycosylated; contains sialic acid. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG66249 anti-Melanoma gp100 antibody [SQab1738] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Melanoma tissue stained with ARG66249 anti-Melanoma gp100 antibody [SQab1738] at 1:200. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
