ARG23870

Name: anti-N Cadherin antibody
Brand: arigo Biolaboratories
SKU: ARG23870
Price: 335 USD
Availability: InStock
Rating: 5.0 (1 reviews)

anti-N Cadherin antibody

Datasheet

参考文献2

订购信息

包装

价格 (USD)

50 μl

335

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes N Cadherin
反应物种	Hu, Ms, Rat, Sheep
预测物种	Chimp, Chk
应用	ELISA, FACS, ICC/IF, IHC-P, IP, WB
特异性	The antibody detects a band at ~ 130 kDa in western blots of Human endothelial cells and Mouse brain tissue, and does not cross-react with E Cadherin.
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	N Cadherin
抗原物种	Human
抗原	Carrier protein coupled synthetic peptide corresponding to aa. 811-824 of Human N Cadherin.
偶联标记	Un-conjugated
別名	Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325

应用说明

应用建议

应用	推荐稀释比
ELISA	1:2000
FACS	1:100
ICC/IF	1:200
IHC-P	1 µg/ml
IP	1:100
WB	1:1000

应用说明

WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

实际分子量

~ 130 kDa

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.
抗菌剂	0.05% Sodium azide.
稳定剂	50% Glycerol and 1 mg/ml BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 1000 Human CDH2 GeneID: 12558 Mouse CDH2 GeneID: 83501 Rat CDH2 Swiss-port # P15116 Mouse Cadherin-2 Swiss-port # P19022 Human Cadherin-2 Swiss-port # Q9Z1Y3 Rat Cadherin-2 More
基因名称	CDH2
全名	cadherin 2, type 1, N-cadherin (neuronal)
背景介绍	N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
生物功能	N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]
细胞定位	Cell membrane; Single-pass type I membrane protein. Cell membrane, sarcolemma. Cell junction. Cell surface. Note=Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. [UniProt]
产品亮点	Related Antibody Duos and Panels: ARG30320 EMT Marker Antibody Panel Related products: N Cadherin antibodies; N Cadherin ELISA Kits; N Cadherin Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New EMT antibody panel is released
研究领域	EMT Study antibody; Mesenchymal Markers antibody
预测分子量	100 kDa
翻译后修饰	Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity). May be phosphorylated by OBSCN. [UniProt]

检测图片 (5) Click the Picture to Zoom In

ARG23870 anti-N Cadherin antibody WB image

Western blot: 20 µg of mouse brain stained with ARG23870 anti-N Cadherin antibody at 1:1000 dilution. (Observed MW: ~130 kDa)
ARG23870 anti-N Cadherin antibody ICC/IF image

Immunofluorescence: MCF7-ADR cells stained with ARG23870 anti-N Cadherin antibody.
ARG23870 anti-N Cadherin antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded human pancreatic adenocarcinoma stained with ARG23870 anti-N Cadherin antibody.
ARG23870 anti-N Cadherin antibody WB image

Western blot: Human endothelial cells untreated (left) or treated with Pervanadate (1 mM) for 30 min (right). The blots were stained with ARG23870 anti-N Cadherin antibody. (Observed MW: ~130 kDa)
ARG23870 anti-N Cadherin antibody IP image

Immunoprecipitation: Mouse brain lysate immunoprecipitated with no antibody (lane 1), ARG23870 anti-N Cadherin antibody (lane 2) and whole mouse brain lysate (lane 3). The blot was stained with anti-N Cadherin (Cytoplasmic) Mouse monoclonal antibody (lanes 1-3).