ARG65570
anti-NDUFAF1 antibody
anti-NDUFAF1 antibody for Western blot and Human
Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NDUFAF1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NDUFAF1 |
| 抗原物种 | Human |
| 抗原 | Fusion protein of human NDUFAF1 |
| 偶联标记 | Un-conjugated |
| 別名 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1; Complex I intermediate-associated protein 30, mitochondrial; CGI-65; CGI65; CIA30 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | 231 and Jurkat cell |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by antigen-affinity chromatography. |
| 缓冲液 | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 40% Glycerol |
| 浓度 | 2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9Y375 Human Complex I intermediate-associated protein 30, mitochondrial |
|---|---|
| 基因名称 | NDUFAF1 |
| 全名 | NADH dehydrogenase (ubiquinone) complex I, assembly factor 1 |
| 背景介绍 | This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. |
| 生物功能 | Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). [UniProt] |
| 产品亮点 | Related products: Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| 研究领域 | Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 38 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG65570 anti-NDUFAF1 antibody WB image
Western blot: 40 μg of 1) 231 and 2) Jurkat cell lysates stained with ARG65570 anti-NDUFAF1 antibody at 1:500 dilution. Exposure time: 20 seconds.
