ARG65261
anti-NDUFS1 antibody
anti-NDUFS1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes NDUFS1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NDUFS1 |
| 抗原物种 | Human |
| 抗原 | C-TEKSATYVNTEGR |
| 偶联标记 | Un-conjugated |
| 別名 | CI-75k; EC 1.6.5.3; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; CI-75Kd; EC 1.6.99.3; PRO1304; Complex I-75kD; CI-75kD |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P28331 Human NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Swiss-port # Q91VD9 Mouse NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial |
|---|---|
| 背景介绍 | The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 79 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG65261 anti-NDUFS1 antibody WB image
Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG65261 anti-NDUFS1 antibody at 0.3 µg/ml dilution.
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ARG65261 anti-NDUFS1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65261 anti-NDUFS1 antibody at 5 µg/ml dilution followed by AP-staining.
