ARG65316

anti-NDUFS7 antibody

anti-NDUFS7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes NDUFS7
反应物种 Hu
预测物种 Ms, Rat, Cow, Dog
应用 IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 NDUFS7
抗原物种 Human
抗原 C-SRGEYVVAKLD
偶联标记 Un-conjugated
別名 EC 1.6.5.3; EC 1.6.99.3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; CI-20; CI-20KD; NADH-ubiquinone oxidoreductase 20 kDa subunit; PSST subunit; CI-20kD; Complex I-20kD; PSST; MY017

应用说明

应用建议
应用 推荐稀释比
IHC-PAssay - dependent
WB1 - 3 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 374291 Human NDUFS7

Swiss-port # O75251 Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

背景介绍 This gene encodes a protein that is a subunit of one of the complexes that forms the mitochondrial respiratory chain. This protein is one of over 40 subunits found in complex I, the nicotinamide adenine dinucleotide (NADH):ubiquinone oxidoreductase. This complex functions in the transfer of electrons from NADH to the respiratory chain, and ubiquinone is believed to be the immediate electron acceptor for the enzyme. Mutations in this gene cause Leigh syndrome due to mitochondrial complex I deficiency, a severe neurological disorder that results in bilaterally symmetrical necrotic lesions in subcortical brain regions. [provided by RefSeq, Jul 2008]
产品亮点 Related products:
Anti-Goat IgG secondary antibodies;
Related poster download:
The Structure & Functions of Mitochondria.pdf
研究领域 Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
预测分子量 24 kDa
翻译后修饰 Hydroxylated ar Arg-111 by NDUFAF5 early in the pathway of assembly of complex I, before the formation of the juncture between peripheral and membrane arms.

检测图片 (3) Click the Picture to Zoom In

  • ARG65316 anti-NDUFS7 antibody WB image

    Western Blot: Human Heart lysate (35µg protein in RIPA buffer) stained with ARG65316 anti-NDUFS7 antibody (0.1µg/ml)

  • ARG65316 anti-NDUFS7 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65316 anti-NDUFS7 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG65316 anti-NDUFS7 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65316 anti-NDUFS7 antibody at 5 µg/ml dilution followed by AP-staining.