ARG42007
anti-NEDD4L / NEDD4-2 phospho (Ser448) antibody
anti-NEDD4L / NEDD4-2 phospho (Ser448) antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NEDD4L / NEDD4-2 phospho (Ser448) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NEDD4L / NEDD4-2 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Ser448 of Human NEDD4L (NP_001138439.1). |
| 偶联标记 | Un-conjugated |
| 別名 | hNEDD4-2; EC 6.3.2.-; NEDD4.2; Nedd4-2; RSP5; E3 ubiquitin-protein ligase NEDD4-like; NEDD4-2 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | NIH/3T3 | ||||
| 实际分子量 | ~ 130 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q96PU5 Human E3 ubiquitin-protein ligase NEDD4-like |
|---|---|
| 基因名称 | NEDD4L |
| 全名 | neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase |
| 背景介绍 | This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| 生物功能 | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. [UniProt] |
| 细胞定位 | Cytoplasm. Golgi apparatus. Endosome, multivesicular body. Note=May be recruited to exosomes by NDFIP1. [UniProt] |
| 预测分子量 | 112 kDa |
| 翻译后修饰 | Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation. Auto-ubiquitinated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
-
ARG42007 anti-NEDD4L / NEDD4-2 phospho (Ser448) antibody WB image
Western blot: 25 µg of NIH/3T3 cell lysate stained with ARG42007 anti-NEDD4L / NEDD4-2 phospho (Ser448) antibody at 1:1000 dilution.
