ARG59010
anti-NEDD8 antibody
anti-NEDD8 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NEDD8 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NEDD8 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 20-60 of Human NEDD8 (TDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYK). |
| 偶联标记 | Un-conjugated |
| 別名 | NEDD8; Ubiquitin-like protein Nedd8; Neddylin; NEDD-8; Neural precursor cell expressed developmentally down-regulated protein 8 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NEDD8 |
| 全名 | neural precursor cell expressed, developmentally down-regulated 8 |
| 生物功能 | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. [UniProt] |
| 细胞定位 | Nucleus. Mainly nuclear. [UniProt] |
| 预测分子量 | 9 kDa |
| 翻译后修饰 | Cleavage of precursor form by UCHL3 or SENP8 is necessary for function. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG59010 anti-NEDD8 antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG59010 anti-NEDD8 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG59010 anti-NEDD8 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG59010 anti-NEDD8 antibody at 1 µg/ml dilution.
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ARG59010 anti-NEDD8 antibody WB image
Western blot: Rat testis, Mouse thymus, Mouse brain, HeLa and MCF-7 lysates stained with ARG59010 anti-NEDD8 antibody at 0.5 µg/ml dilution.
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ARG59010 anti-NEDD8 antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59010 anti-NEDD8 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
