ARG40442
anti-NEU1 / Neuraminidase antibody
anti-NEU1 / Neuraminidase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NEU1 / Neuraminidase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NEU1 / Neuraminidase |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 156-415 of Human NEU1 (NP_000425.1). |
| 偶联标记 | Un-conjugated |
| 別名 | NANH; Acetylneuraminyl hydrolase; SIAL1; Lysosomal sialidase; EC 3.2.1.18; N-acetyl-alpha-neuraminidase 1; Sialidase-1; NEU; G9 sialidase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse liver | ||||||
| 实际分子量 | 48 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NEU1 |
| 全名 | sialidase 1 (lysosomal sialidase) |
| 背景介绍 | The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage. [UniProt] |
| 细胞定位 | Lysosome membrane; Peripheral membrane protein; Lumenal side. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Lysosome. Note=Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles. [UniProt] |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | N-glycosylated. Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG40442 anti-NEU1 / Neuraminidase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG40442 anti-NEU1 / Neuraminidase antibody at 1:100 dilution.
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ARG40442 anti-NEU1 / Neuraminidase antibody WB image
Western blot: 25 µg of Mouse liver lysate stained with ARG40442 anti-NEU1 / Neuraminidase antibody at 1:1000 dilution.
