ARG59418
anti-NLRC4 / CARD12 antibody
anti-NLRC4 / CARD12 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
NLRC4 Inflammasome Study antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NLRC4 / CARD12 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NLRC4 / CARD12 |
| 抗原物种 | Mouse |
| 抗原 | Recombinant fusion protein corresponding to aa. 89-159 of Mouse NLRC4 (NP_001028539.1). |
| 偶联标记 | Un-conjugated |
| 別名 | CLAN; AIFEC; IPAF; CLANA; CARD12; CLANC; CLANB; CLAND; NLR family CARD domain-containing protein 4; Caspase recruitment domain-containing protein 12; Ipaf; CLR2.1; CARD, LRR, and NACHT-containing protein; CLAN1; Ice protease-activating factor; Clan protein; FCAS4 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse spleen and U87 | ||||||||
| 实际分子量 | 116 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q3UP24 Mouse NLR family CARD domain-containing protein 4 Swiss-port # Q9NPP4 Human NLR family CARD domain-containing protein 4 |
|---|---|
| 基因名称 | NLRC4 |
| 全名 | NLR family, CARD domain containing 4 |
| 背景介绍 | NLRC4 / CARD12 is a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| 生物功能 | NLRC4 / CARD12 is a key component. of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. [UniProt] |
| 细胞定位 | Cytoplasm. Cytoplasm, cytosol. Note=Cytoplasmic filaments. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30332 NLRC4 Inflammasome Antibody Panel Related products: NLRC4 antibodies; NLRC4 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | NLRC4 Inflammasome Study antibody |
| 预测分子量 | 116 kDa |
| 翻译后修饰 | Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro (By similarity). [UniProt] |
检测图片 (6) Click the Picture to Zoom In
-
ARG59418 anti-NLRC4 / CARD12 antibody ICC/IF image
Immunofluorescence: THP-1 cells stained with ARG59418 anti-NLRC4 / CARD12 antibody (red).
-
ARG59418 anti-NLRC4 / CARD12 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung stained with ARG59418 anti-NLRC4 / CARD12 antibody at 1:100 dilution (40x lens).
-
ARG59418 anti-NLRC4 / CARD12 antibody WB image
Western blot: 25 µg of Mouse spleen and U87 cell lysate stained with ARG59418 anti-NLRC4 / CARD12 antibody at 1:3000 dilution.
-
ARG59418 anti-NLRC4 / CARD12 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon stained with ARG59418 anti-NLRC4 / CARD12 antibody at 1:100 dilution (40x lens).
-
ARG59418 anti-NLRC4 / CARD12 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric stained with ARG59418 anti-NLRC4 / CARD12 antibody at 1:100 dilution (40x lens).
-
ARG59418 anti-NLRC4 / CARD12 antibody IHC-P image
Immunohistochemistry: Mouse lung stained with ARG59418 anti-NLRC4 / CARD12 antibody (orange-red).
