ARG22881
anti-Nestin antibody [Rat-401 (4D4)]
anti-Nestin antibody [Rat-401 (4D4)] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [Rat-401 (4D4)] recognizes Nestin Mouse anti Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. |
|---|---|
| 反应物种 | Ms, Rat |
| 不反应物种 | Hu |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | Rat-401 (4D4) |
| 同位型 | IgG1 |
| 靶点名称 | Nestin |
| 抗原物种 | Rat |
| 抗原 | Nestin purified from embryonic Rat spinal cord. |
| 偶联标记 | Un-conjugated |
| 別名 | NES; Nestin FLJ21841; Nbla00170 |
应用说明
| 应用建议 |
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| 应用说明 | IHC: We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5. WB: Mouse anti Rat Nestin antibody, clone Rat-401 reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts. For western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Unpurified. |
| 缓冲液 | Tris-buffered saline and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NES |
| 全名 | Nestin |
| 背景介绍 | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. |
| 生物功能 | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| 细胞定位 | Intermediate filament |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody |
| 预测分子量 | 177 kDa |
| 翻译后修饰 | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation |
