ARG53358

anti-Nestin antibody [SP103]

anti-Nestin antibody [SP103] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
publication_link 参考文献1

概述

产品描述 Rabbit Monoclonal antibody [SP103] recognizes Nestin
反应物种 Hu
应用 IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SP103
同位型 IgG
靶点名称 Nestin
抗原物种 Human
抗原 Synthetic peptide corresponding to C-terminus of human nestin.
偶联标记 Un-conjugated
別名 NES; Nestin FLJ21841; Nbla00170

应用说明

应用建议
应用 推荐稀释比
IHC-P1:100
WB1:2000
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Kidney, HeLa Cell Lysate

属性

形式 Liquid
纯化 Purified by protein A/G
缓冲液 PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
抗菌剂 < 0.1% Sodium azide
稳定剂 1% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10763 Human NES

Swiss-port # P48681 Human Nestin

基因名称 NES
全名 nestin
背景介绍 This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.
生物功能 Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
细胞定位 Intermediate filament
产品亮点 Related Antibody Duos and Panels:
ARG30083 Neuron Development Marker Antibody Duo (Nestin, Tau)
Related products:
Nestin antibodies; Nestin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
预测分子量 177 kDa
翻译后修饰 Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

检测图片 (11) Click the Picture to Zoom In

  • ARG53358 anti-Nestin antibody [SP103] WB image

    Western Blot: HeLa stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Lung stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Prostate stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Kidney stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Skin stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Ovary stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Cervix stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Breast stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Colon stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Endometrium stained with Nestin antibody [SP103] (ARG53358)

  • ARG53358 anti-Nestin antibody [SP103] IHC-P image

    Immunohistochemistry: Human Cerebellum stained with Nestin antibody [SP103] (ARG53358)

文献引用

In Vivo Melanoma Cell Morphology Reflects Molecular Signature and Tumor Aggressiveness

IHC-P / Human

Alessandra Marconi et al.
J Invest Dermatol.,  (2022)

publication_link

 

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