ARG43290

anti-Neurotrophin 3 antibody

anti-Neurotrophin 3 antibody for Flow cytometry,Western blot and Human,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes Neurotrophin 3
反应物种 Hu, Rat
预测物种 Ms
应用 FACS, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Neurotrophin 3
抗原物种 Human
抗原 KLH-conjugated synthetic peptide between aa. 152-184 of Human Neurotrophin 3.
偶联标记 Un-conjugated
別名 Nerve growth factor 2; NT3; HDNF; Neurotrophic factor; Neurotrophin-3; NT-3; NGF2; NGF-2

应用说明

应用建议
应用 推荐稀释比
FACS1:25
WB1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human ovary
实际分子量 ~ 27 kDa

属性

形式 Liquid
纯化 Purification with Protein A and immunogen peptide.
缓冲液 PBS and 0.09% (W/V) Sodium azide.
抗菌剂 0.09% (W/V) Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4908 Human NTF3

GeneID: 81737 Rat NTF3

Swiss-port # P18280 Rat Neurotrophin-3

Swiss-port # P20783 Human Neurotrophin-3

基因名称 NTF3
全名 neurotrophin 3
背景介绍 The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]
生物功能 Seems to promote the survival of visceral and proprioceptive sensory neurons. [UniProt]
细胞定位 Secreted. [UniProt]
预测分子量 29 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG43290 anti-Neurotrophin 3 antibody WB image

    Western blot: 20 µg of Human ovary lysate stained with ARG43290 anti-Neurotrophin 3 antibody at 1:2000 dilution.

  • ARG43290 anti-Neurotrophin 3 antibody FACS image

    Flow Cytometry: HeLa cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. Cells were incubated in 2% BSA to block non-specific protein-protein interactions. Cells were stained with ARG43290 anti-Neurotrophin 3 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was rabbit IgG1 (1 µg/10^6 cells) used under the same conditions.