ARG55186
anti-OPN / Osteopontin antibody
anti-OPN / Osteopontin antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes OPN / Osteopontin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | OPN / Osteopontin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (18 aa) within aa. 50-100 of Human OPN / Osteopontin. |
| 偶联标记 | Un-conjugated |
| 別名 | BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human Bladder Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A purified. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SPP1 |
| 全名 | secreted phosphoprotein 1 |
| 背景介绍 | The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts. SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells. SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite and the growth of calcium oxalate crystals. |
| 生物功能 | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt] |
| 产品亮点 | Related products: OPN antibodies; OPN ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 35 kDa |
| 翻译后修饰 | Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
检测图片 (3) Click the Picture to Zoom In
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ARG55186 anti-OPN / Osteopontin antibody IHC image
Immunohistochemistry: SPP1 in Human bladder tissue stained with ARG55186 anti-OPN / Osteopontin antibody at 20 ug/ml dilution.
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ARG55186 anti-OPN / Osteopontin antibody WB image
Western blot: Human bladder tissue lysate stained with ARG55186 anti-OPN / Osteopontin antibody at (A) 1 and (B) 2 ug/ml dilution.
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ARG55186 anti-OPN / Osteopontin antibody IHC image
Immunohistochemistry: Human bladder tissue stained with ARG55186 anti-OPN / Osteopontin antibody at 5 ug/ml dilution.
