ARG58805
anti-OPN / Osteopontin antibody
anti-OPN / Osteopontin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes OPN / Osteopontin |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | OPN / Osteopontin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 28-47 of Human OPN / Osteopontin (EEKQLYNKYPDAVATWLNPD). |
| 偶联标记 | Un-conjugated |
| 別名 | BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SPP1 |
| 全名 | secreted phosphoprotein 1 |
| 背景介绍 | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| 生物功能 | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: OPN antibodies; OPN ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| 预测分子量 | 35 kDa |
| 翻译后修饰 | Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58805 anti-OPN / Osteopontin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer stained with ARG58805 anti-OPN / Osteopontin antibody.
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ARG58805 anti-OPN / Osteopontin antibody WB image
Western blot: MDA-MB-231, HeLa, Jurkat and COLO320 cell lysates stained with ARG58805 anti-OPN / Osteopontin antibody.
