ARG53386
anti-Occludin antibody
anti-Occludin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Signaling Transduction antibody
参考文献3
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Occludin |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Occludin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to C-terminus of human occludin. |
| 偶联标记 | Un-conjugated |
| 別名 | PPP1R115; BLCPMG; Occludin |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Lung, Human Kidney |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunogen affinity purified |
| 缓冲液 | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| 抗菌剂 | < 0.1% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | OCLN |
| 全名 | occludin |
| 背景介绍 | This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011] |
| 生物功能 | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. [UniProt] |
| 细胞定位 | Membrane |
| 产品亮点 | Related products: Occludin antibodies; Occludin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 59 kDa |
| 翻译后修饰 | Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1. |
检测图片 (2) Click the Picture to Zoom In
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ARG53386 anti-Occludin antibody WB image
Western Blot: Kidney stained with Occludin antibody (ARG53386)
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ARG53386 anti-Occludin antibody IHC-P image
Immunohistochemistry: Human Lung stained with Occludin antibody (ARG53386)
客户反馈
Average
anti-Occludin antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-Occludin antibody
Application：WB
Sample：Mouse kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Study of Magnesium Formulations on Intestinal Cells to Influence Myometrium Cell Relaxation.
WB / Human
Meningitic Escherichia coli-induced upregulation of PDGF-B and ICAM-1 aggravates blood-brain barrier disruption and neuroinflammatory response.
WB, IHC-P / Human, Mouse
Induction of VEGFA and Snail-1 by meningitic Escherichia coli mediates disruption of the blood-brain barrier.
IHC-P / Mouse
