ARG54605
anti-Osteocalcin antibody [OC4-30]
anti-Osteocalcin antibody [OC4-30] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Chicken,Dog,Goat,Pig,Rabbit,Sheep
Developmental Biology antibody; Signaling Transduction antibody
参考文献10
概述
| 产品描述 | Mouse Monoclonal antibody [OC4-30] recognizes Osteocalcin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov, Chk, Dog, Goat, Pig, Rb, Sheep |
| 应用 | ELISA, ICC/IF, IHC-Fr, IHC-P, WB |
| 特异性 | Includes the γ-carboxylated residue at position 17 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | OC4-30 |
| 同位型 | IgG2a |
| 靶点名称 | Osteocalcin |
| 抗原物种 | Bovine |
| 抗原 | Bovine osteocalcin |
| 偶联标记 | Un-conjugated |
| 別名 | OCN; Gamma-carboxyglutamic acid-containing protein; Osteocalcin; OC; Bone Gla protein; BGP |
应用说明
| 应用说明 | Sandwich ELISA: 1 - 10 ug/ml. Western blot: 1 - 10 ug/ml, reducing or non-reducing conditions. Immunohistochemistry: 1 - 10 ug/ml, paraffin-embedded or frozen tissue sections. Antigen retrieval: Proteinase K (0.4 mg/ml) at RT for 5 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 10 mM PBS (pH 7.4) and 1% BSA |
| 稳定剂 | 1% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BGLAP |
| 全名 | bone gamma-carboxyglutamate (gla) protein |
| 背景介绍 | This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015] |
| 生物功能 | Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. [UniProt] |
| 研究领域 | Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 11 kDa |
| 翻译后修饰 | Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. |
检测图片 (2) Click the Picture to Zoom In
-
ARG54605 anti-Osteocalcin antibody [OC4-30] IHC-P image
Immunohistochemistry: Parrafin-embedded chondroma tissue stained with ARG54605 anti-Osteocalcin antibody [OC4-30] at 1:200 (10 µg/ml) dilution, RT for 60 min. Antigen retrieval: Proteinase K (0.4 mg/ml) at RT for 5 min.
-
ARG54605 anti-Osteocalcin antibody [OC4-30] IHC-P image
Immunohistochemistry: Parrafin-embedded synovial sarcoma tissue stained with ARG54605 anti-Osteocalcin antibody [OC4-30] at 1:200 (10 µg/ml) dilution, RT for 60 min. Antigen retrieval: Proteinase K (0.4 mg/ml) at RT for 5 min.
文献引用
Bone Marrow Mononuclear Cell Transplantation Promotes Bone Healing via Gap Junction-Mediated Cell-Cell Interaction
ICC/IF / Mouse
Surface Crystal and Degradability of Shape Memory Scaffold Essentialize Osteochondral Regeneration
IHC-P / Rat
Liraglutide in Combination with Insulin Has a Superior Therapeutic Effect to Either Alone on Fracture Healing in Diabetic Rats
WB / IHC-P / Rat
Ablation of Discoidin Domain Receptor 1 Provokes an Osteopenic Phenotype by Regulating Osteoblast/Osteocyte Autophagy and Apoptosis
IHC-P / Mouse
Inhibiting PHD2 in human periodontal ligament cells via lentiviral vector-mediated RNA interference facilitates cell osteogenic differentiation and periodontal repair
WB / Human
Simvastatin stimulates osteogenic differentiation of bone marrow mesenchymal stem cells.
ICC/IF / Mouse
Peripheral sensory nerves regulate orthodontic tooth movement-induced bone formation via Wnt5a signaling pathway.
IHC-P / Rat
Identification of Gli1-interacting proteins during simvastatin-stimulated osteogenic differentiation of bone marrow mesenchymal stem cells.
WB, ICC/IF / Rat
Stiffness Modification of Photopolymerizable Gelatin-Methacrylate Hydrogels Influences Endothelial Differentiation of Human Mesenchymal Stem Cells.
ICC/IF / Human
Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats.
WB / Rat
