ARG42665
anti-PAK3 antibody
anti-PAK3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PAK3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 特异性 | ARG42665 anti-PAK3 antibody reacts to PAK1 with low binding affinity and not react to PAK2. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PAK3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 1-50 of Human PAK3. |
| 偶联标记 | Un-conjugated |
| 別名 | beta-PAK; bPAK; MRX30; PAK3beta; OPHN3; Oligophrenin-3; MRX47; Serine/threonine-protein kinase PAK 3; EC 2.7.11.1; PAK-3; p21-activated kinase 3; Beta-PAK |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Human fetal brain | ||||||
| 实际分子量 | ~ 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PAK3 |
| 全名 | p21 protein (Cdc42/Rac)-activated kinase 3 |
| 背景介绍 | The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017] |
| 生物功能 | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | Autophosphorylated when activated by CDC42/p21. Neddylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG42665 anti-PAK3 antibody WB image
Western blot: Human fetal brain lysate stained with ARG42665 anti-PAK3 antibody.
