ARG55239

anti-PAX6 antibody

anti-PAX6 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes PAX6
反应物种 Hu
预测物种 Ms, Rat, Bov, Xenopus
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PAX6
抗原物种 Human
抗原 KLH-conjugated synthetic peptide corresponding to aa. 183-210 (Center) of Human PAX6.
偶联标记 Un-conjugated
別名 MGDA; Aniridia type II protein; AN; Oculorhombin; Paired box protein Pax-6; AN2; WAGR; FVH1; D11S812E

应用说明

应用建议
应用 推荐稀释比
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 U251

属性

形式 Liquid
纯化 Purification with Protein A and immunogen peptide.
缓冲液 PBS and 0.09% (W/V) Sodium azide
抗菌剂 0.09% (W/V) Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 5080 Human PAX6

Swiss-port # P26367 Human Paired box protein Pax-6

基因名称 PAX6
全名 paired box 6
背景介绍 This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
生物功能 Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes. [UniProt]
细胞定位 Nucleus.
产品亮点 Related products:
PAX6 antibodies; Anti-Rabbit IgG secondary antibodies;
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研究领域 Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
预测分子量 47 kDa
翻译后修饰 Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.

检测图片 (4) Click the Picture to Zoom In

  • ARG55239 anti-PAX6 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG55239 anti-PAX6 antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

  • ARG55239 anti-PAX6 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG55239 anti-PAX6 antibody.

  • ARG55239 anti-PAX6 antibody WB image

    Western blot: 35 µg of U251 cell lysate stained with ARG55239 anti-PAX6 antibody.

  • ARG55239 anti-PAX6 antibody FACS image

    Flow Cytometry: HeLa cells stained with ARG55239 anti-PAX6 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.