ARG41101
anti-PDGF BB antibody
anti-PDGF BB antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PDGF BB |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Cow, Dog, Gpig, Sheep |
| 应用 | IHC-P, WB |
| 特异性 | This antibody react to full length and proform of PDGF BB but not mature form of PDGF B. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PDGF BB |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the N-terminal region (a.a. 2-51) of Human PDGF B. (within the following region: NRCWALFLSLCCYLRLVSAEGDPIPEELYEMLSDHSIRSFDDLQRLLHGD) |
| 偶联标记 | Un-conjugated |
| 別名 | SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain |
应用说明
| 预测反应性说明 | Predicted Homology Based On Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea pig: 100%; Sheep: 100% | ||||||
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| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | 293T | ||||||
| 实际分子量 | 31 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 0.09% (w/v) Sodium azide and 2% Sucrose. |
| 抗菌剂 | 0.09% (w/v) Sodium azide |
| 稳定剂 | 2% Sucrose |
| 浓度 | Batch dependent: 0.5 - 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PDGFB |
| 全名 | platelet-derived growth factor beta polypeptide |
| 背景介绍 | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013] |
| 生物功能 | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity). [UniProt] |
| 细胞定位 | Secreted. Note=Released by platelets upon wounding. [UniProt] |
| 预测分子量 | 27 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG41101 anti-PDGF B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue stained with ARG41101 anti-PDGF B antibody at 5 µg/ml dilution.
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ARG41101 anti-PDGF B antibody WB image
Western blot: 293T cell lysate stained with ARG41101 anti-PDGF B antibody at 0.2 - 1 µg/ml dilution.
