ARG55419
anti-PINK1 antibody [38CT20.8.5]
anti-PINK1 antibody [38CT20.8.5] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes PINK1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 38CT20.8.5 |
| 同位型 | IgG1 |
| 靶点名称 | PINK1 |
| 抗原物种 | Human |
| 抗原 | Recombinant Human PINK1 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | PARK6; BRPK; PTEN-induced putative kinase protein 1; Serine/threonine-protein kinase PINK1, mitochondrial; EC 2.7.11.1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | A431 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9BXM7 Human Serine/threonine-protein kinase PINK1, mitochondrial |
|---|---|
| 基因名称 | PINK1 |
| 全名 | PTEN induced putative kinase 1 |
| 背景介绍 | This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008] |
| 生物功能 | Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form. [UniProt] |
| 细胞定位 | Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm, cytosol |
| 产品亮点 | Related products: PINK1 antibodies; Anti-Mouse IgG secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 63 kDa |
| 翻译后修饰 | Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria. Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol. |
检测图片 (2) Click the Picture to Zoom In
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ARG55419 anti-PINK1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart section stained with ARG55419 anti-PINK1 antibody at 1:25 dilution.
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ARG55419 anti-PINK1 antibody WB image
Western blot: 20 µg of A431 cell lysate stained with ARG55419 anti-PINK1 antibody at 1:1000 dilution.
