ARG61996
anti-PKM1 / 2 antibody
anti-PKM1 / 2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Colorectal Carcinoma Marker antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PKM1 / 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PKM1 / 2 |
| 抗原物种 | Human |
| 抗原 | Recombinant Protein of human PKM2 |
| 偶联标记 | Un-conjugated |
| 別名 | PK3; PKM2; OIP3; Pyruvate kinase muscle isozyme; CTHBP; HEL-S-30; THBP1; OIP-3; Pyruvate kinase 2/3; Tumor M2-PK; Cytosolic thyroid hormone-binding protein; EC 2.7.1.40; Opa-interacting protein 3; p58; TCB; Pyruvate kinase PKM; Thyroid hormone-binding protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were purified by affinity purification using immunogen. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PKM |
| 全名 | pyruvate kinase, muscle |
| 背景介绍 | Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1). Studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1). When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 (4). A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice (4). These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (4). |
| 生物功能 | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30013 Colorectal Carcinoma Marker Antibody Duo (Colorectal carcinoma antibody, PKM2) ARG30014 Phospho PKM2 Antibody Duo (Total, pS37) Related products: PKM antibodies; PKM Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Colorectal Carcinoma Marker antibody |
| 预测分子量 | 58 kDa |
| 翻译后修饰 | ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. |
检测图片 (5) Click the Picture to Zoom In
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ARG61996 anti-PKM1/2 antibody WB image
Western blot: 30 µg of Jurkat, A549, and U2OS cell lysates stained with ARG61996 anti-PKM1/2 antibody at 1:500 dilution.
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ARG61996 anti-PKM1/2 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG61996 anti-PKM1/2 antibody at 1:20 dilution. Left: primary antibody (red). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
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ARG61996 anti-PKM1/2 antibody IHC-P image
Immunohistochemistry: paraffin-embedded Lung cancer stained with anti-PKM1/2 antibody ARG61996.
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ARG61996 anti-PKM1/2 antibody IHC-P image
Immunohistochemistry: paraffin-embedded mouse lung stained with ARG61996 anti-PKM1/2 antibody at dilution of 1:100 (400x lens).
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ARG61996 anti-PKM1/2 antibody IHC-P image
Immunohistochemistry: paraffin-embedded rat kidney stained with ARG61996 anti-PKM1/2 antibody at dilution of 1:100 (200x lens).
客户反馈
Excellent
anti-PKM1 / 2 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:20
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
anti-PKM1 / 2 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
