ARG57138

anti-PKM2 antibody [1B10]

anti-PKM2 antibody [1B10] for ICC/IF,Western blot and Human

Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Colorectal Carcinoma Marker antibody

概述

产品描述 Mouse Monoclonal antibody [1B10] recognizes PKM2
反应物种 Hu
应用 ICC/IF, WB
宿主 Mouse
克隆 Monoclonal
克隆号 1B10
同位型 IgG1, kappa
靶点名称 PKM2
抗原物种 Human
抗原 Recombinant fragment around aa. 1-531 of Human PKM2
偶联标记 Un-conjugated
別名 PK3; PKM2; OIP3; Pyruvate kinase muscle isozyme; CTHBP; HEL-S-30; THBP1; OIP-3; Pyruvate kinase 2/3; Tumor M2-PK; Cytosolic thyroid hormone-binding protein; EC 2.7.1.40; Opa-interacting protein 3; p58; TCB; Pyruvate kinase PKM; Thyroid hormone-binding protein 1

应用说明

应用建议
应用 推荐稀释比
ICC/IFAssay-dependent
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 10% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 5315 Human PKM

Swiss-port # P14618 Human Pyruvate kinase PKM

基因名称 PKM
全名 pyruvate kinase, muscle
背景介绍 This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
生物功能 Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt]
产品亮点 Related products:
PKM2 antibodies; PKM2 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Vascular development is regulated by FGF-dependent metabolic control
研究领域 Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Colorectal Carcinoma Marker antibody
预测分子量 58 kDa
翻译后修饰 ISGylated.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.

检测图片 (4) Click the Picture to Zoom In

  • ARG57138 anti-PKM2 antibody [1B10] ICC/IF image

    Immunofluorescence: HeLa cells line stained with ARG57138 anti-PKM2 antibody [1B10] at 1:100 (Green).

    DAPI (Blue) for nucleus staining.

  • ARG57138 anti-PKM2 antibody [1B10] WB image

    Western blot: 40 µg of 1) HeLa, 2) Jurkat, 3) MCF7, 4) A549, and 5) 293T cell lysates stained with ARG57138 anti-PKM2 antibody [1B10] at 1:1000.

  • ARG57138 anti-PKM2 antibody [1B10] ICC/IF image

    Immunofluorescence: A549 cells line stained with ARG57138 anti-PKM2 antibody [1B10] at 1:100 (Green).

    DAPI (Blue) for nucleus staining.

  • ARG57138 anti-PKM2 antibody [1B10] WB image

    Western blot: 50 ng of Recombinant Human PKM2 protein stained with ARG57138 anti-PKM2 antibody [1B10] at 1:1000.