ARG40971
anti-PLA2G7 / Lp-PLA2 antibody
anti-PLA2G7 / Lp-PLA2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PLA2G7 / Lp-PLA2 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PLA2G7 / Lp-PLA2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 200-228 of Human PLA2G7. |
| 偶联标记 | Un-conjugated |
| 別名 | LDL-PLA; gVIIA-PLA2; LDL-PLA2; Group-VIIA phospholipase A2; 2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1.47; LDL-associated phospholipase A2; PAF 2-acylhydrolase; 2; PAF acetylhydrolase; PAFAH; Platelet-activating factor acetylhydrolase; PAFAD; 1-alkyl-2-acetylglycerophosphocholine esterase; LP-PLA2 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | HL-60 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13093 Human Platelet-activating factor acetylhydrolase |
|---|---|
| 基因名称 | PLA2G7 |
| 全名 | phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) |
| 背景介绍 | The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2009] |
| 生物功能 | Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. [UniProt] |
| 细胞定位 | Secreted, extracellular space. [UniProt] |
| 预测分子量 | 50 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG40971 anti-PLA2G7 / Lp-PLA2 antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human colon tissue. Tissue was blocked with 3% BSA for 0.5 hour at room temperature. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG40971 anti-PLA2G7 / Lp-PLA2 antibody at 1:25 dilution for 1 hours at 37°C.
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ARG40971 anti-PLA2G7 / Lp-PLA2 antibody WB image
Western blot: 35 µg of HL-60 cell lysate stained with ARG40971 anti-PLA2G7 / Lp-PLA2 antibody.
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ARG40971 anti-PLA2G7 / Lp-PLA2 antibody FACS image
Flow Cytometry: HL-60 cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by ARG40971 anti-PLA2G7 / Lp-PLA2 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
