ARG41204
anti-PLC gamma 1 phospho (Ser1248) antibody
anti-PLC gamma 1 phospho (Ser1248) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PLC gamma 1 phospho (Ser1248) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PLC gamma 1 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Ser1248 of Human PLC gamma 1 (NP_002651.2). |
| 偶联标记 | Un-conjugated |
| 別名 | PLC-gamma-1; Phospholipase C-gamma-1; PLC148; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; Phosphoinositide phospholipase C-gamma-1; NCKAP3; PLC-148; Phospholipase C-II; PLC1; PLCgamma1; PLC-II; EC 3.1.4.11 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Jurkat + PMA | ||||||
| 实际分子量 | ~ 150 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PLCG1 |
| 全名 | phospholipase C, gamma 1 |
| 背景介绍 | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. [UniProt] |
| 细胞定位 | Cell projection, lamellipodium. Cell projection, ruffle. Note=Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. [UniProt] |
| 预测分子量 | 149 kDa |
| 翻译后修饰 | Tyrosine phosphorylated in response to signaling via activated FLT3, KIT and PDGFRA (By similarity). Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated by activated FLT1 and KDR. Tyrosine phosphorylated by activated PDGFRB. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases, in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. May be dephosphorylated by PTPRJ. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells. Ubiquitinated by CBLB in activated T-cells. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG41204 anti-PLC gamma 1 phospho (Ser1248) antibody WB image
Western blot: Jurkat cells were treated by PMA/TPA (200 nM) at 37°C for 10 minutes or untreated. 25 µg of cell lysate stained with ARG41204 anti-PLC gamma 1 phospho (Ser1248) antibody.
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ARG41204 anti-PLC gamma 1 phospho (Ser1248) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG41204 anti-PLC gamma 1 phospho (Ser1248) antibody at 1:100 dilution.
