ARG43833

anti-POLD1 antibody

anti-POLD1 antibody for ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes POLD1.
反应物种 Hu, Ms, Rat
应用 ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 DNA Polymerase Delta 1, Catalytic Subunit
抗原 Synthetic peptide of human PolD1.
偶联标记 Un-conjugated
別名 MDPL; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; CRCS10; POLD; CDC2; EC 2.7.7.7

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50-1:200
WB1:500-1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity Purified
缓冲液 Tris-Glycine (pH 7.4) with 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 18971 Mouse POLD1

GeneID: 5424 Human POLD1

GeneID: 59294 Rat POLD1

基因名称 POLD1
全名 polymerase (DNA directed), delta 1, catalytic subunit
背景介绍 This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]
生物功能 Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG43833 anti-POLD1 antibody WB image

    Western blot: A562, C6 3T3 and Hela stained with ARG43833 anti-POLD1 antibody.

  • ARG43833 anti-POLD1 antibody ICC/IF image

    Immunofluorescence: K562 were stained with ARG43833 anti-POLD1 antibody.