ARG43912
anti-PPAN antibody
anti-PPAN antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PPAN |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PPAN |
| 抗原物种 | Human |
| 抗原 | Human PPAN recombinant protein |
| 表达系统 | E.coli |
| 偶联标记 | Un-conjugated |
| Protein Full name | Suppressor of SWI4 1 homolog |
| 別名 | PPAN; Peter Pan Homolog; BXDC3; SSF1; SSF2; SSF; Brix Domain-Containing Protein 3; Suppressor Of SWI4 1 Homolog; Peter Pan (Drosophila) Homolog; Peter Pan Homolog (Drosophila); Homolog Of S. Cerevisiae SSF1; Second-Step Splicing Factor 1; Suppressor Of Sterile Four 1; SSF-1; Ssf-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified with Immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PPAN |
| 全名 | Peter Pan Homolog |
| 背景介绍 | The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation. |
| 生物功能 | A chimeric transcript, characterized by the first third of PPAN exon 12 joined to P2RY11 exon 2, has been detected. It is possibly produced by trans-splicing. The chimeric transcript is widely expressed and can be induced by retinoic acid during the granulocytic differentiation of the HL-60 cell line. The resulting chimeric protein shows a much lower activity than the non-chimeric P2RY11 gene product, but qualitatively indistinguishable. |
| 细胞定位 | Nucleus |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Acetylation, Phosphoprotein |
检测图片 (3) Click the Picture to Zoom In
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ARG43912 anti-PPAN antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG43912 anti-PPAN antibody at 5 μg/ml dilution.
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ARG43912 anti-PPAN antibody WB image
Western blot:Hela and 293T cell stained with ARG43912 anti-PPAN antibody at 0.5 μg/mL dilution.
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ARG43912 anti-PPAN antibody FACS image
Flow Cytometry: JK cells stained with ARG43912 anti-PPAN antibody (blue) at 1 μg/1x10^6 cells dilution.
