ARG59786
anti-PRPF3 antibody
anti-PRPF3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PRPF3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PRPF3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-280 of Human PRPF3 (NP_004689.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Pre-mRNA-splicing factor 3; Prp3p; SNRNP90; U4/U6 small nuclear ribonucleoprotein Prp3; PRP3; hPrp3; U4/U6 snRNP 90 kDa protein; HPRP3; HPRP3P; RP18 |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Mouse testis and BT-474 | ||||||||||
| 实际分子量 | 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O43395 Human U4/U6 small nuclear ribonucleoprotein Prp3 Swiss-port # Q922U1 Mouse U4/U6 small nuclear ribonucleoprotein Prp3 |
|---|---|
| 基因名称 | PRPF3 |
| 全名 | pre-mRNA processing factor 3 |
| 背景介绍 | The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq, Jul 2008] |
| 生物功能 | Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. [UniProt] |
| 细胞定位 | Nucleus speckle. Note=Colocalizes with spliceosomal snRNPs. [UniProt] |
| 产品亮点 | Related products: PRPF3 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Gene therapy for retinitis pigmentosa (RP) |
| 预测分子量 | 78 kDa |
| 翻译后修饰 | Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
-
ARG59786 anti-PRPF3 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG59786 anti-PRPF3 antibody.
-
ARG59786 anti-PRPF3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG59786 anti-PRPF3 antibody at 1:100 dilution.
-
ARG59786 anti-PRPF3 antibody WB image
Western blot: 25 µg of Mouse testis and BT-474 cell lysates stained with ARG59786 anti-PRPF3 antibody at 1:1000 dilution.
-
ARG59786 anti-PRPF3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG59786 anti-PRPF3 antibody at 1:100 dilution.
