ARG66349
anti-PSAP antibody [SQab1899]
anti-PSAP antibody [SQab1899] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab1899] recognizes PSAP |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab1899 |
| 同位型 | IgG |
| 靶点名称 | PSAP |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 1-100 of Human PSAP. |
| 偶联标记 | Un-conjugated |
| 別名 | Glucosylceramidase activator; SAP-1; Sphingolipid activator protein 1; Component C; Protein C; Proactivator polypeptide; Protein A; Sphingolipid activator protein 2; Cerebroside sulfate activator; A1 activator; Prosaposin; Dispersin; SAP-2; Co-beta-glucosidase; CSAct; SAP1; GLBA; Sulfatide/GM1 activator |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PSAP |
| 全名 | prosaposin |
| 背景介绍 | This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| 生物功能 | Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases. Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12). Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling. Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases. [UniProt] |
| 产品亮点 | Related products: PSAP antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| 预测分子量 | 58 kDa |
| 翻译后修饰 | The lysosomal precursor is proteolytically processed to 4 small peptides, which are similar to each other and are sphingolipid hydrolase activator proteins. N-linked glycans show a high degree of microheterogeneity. The one residue extended Saposin-B-Val is only found in 5% of the chains. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG66349 anti-PSAP antibody [SQab1899] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate cancer tissue stained with ARG66349 anti-PSAP antibody [SQab1899]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
