ARG23364
anti-Perforin antibody [B-D48] (PE)
anti-Perforin antibody [B-D48] (PE) for Flow cytometry and Human
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [B-D48] recognizes Perforin |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | This antibody recognizes human Perforin, a 70 kDa protein. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | B-D48 |
| 同位型 | IgG1 |
| 靶点名称 | Perforin |
| 抗原物种 | Human |
| 抗原 | Natural human Perforin |
| 偶联标记 | PE |
| 別名 | FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl to label 10^6 cells or 100 µl of whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | PBS, 0.09% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 5% BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PRF1 |
| 全名 | perforin 1 (pore forming protein) |
| 背景介绍 | Perforin with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] |
| 生物功能 | Perforin plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt] |
| 产品亮点 | Related products: Perforin antibodies; Perforin ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: Examining CTL/NK-mediated cytotoxicity by ELISA |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | N-glycosylated. [UniProt] |
