ARG66673
anti-Podoplanin antibody [SQab19158]
anti-Podoplanin antibody [SQab19158] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab19158] recognizes Podoplanin |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab19158 |
| 同位型 | IgG |
| 靶点名称 | Podoplanin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 1-100 of Human Podoplanin. |
| 偶联标记 | Un-conjugated |
| 別名 | T1A2; T1A; GP36; Aggrus; Gp38; T1A-2; Glycoprotein 36; PA2.26 antigen; TI1A; AGGRUS; HT1A-1; OTS8; Gp36; PA2.26; T1-alpha; Podoplanin; GP40 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PDPN |
| 全名 | podoplanin |
| 背景介绍 | This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. [UniProt] |
| 细胞定位 | Podoplanin: Membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell projection, filopodium membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Membrane raft. Apical cell membrane. Basolateral cell membrane. Cell projection, invadopodium. [UniProt] |
| 预测分子量 | 17 kDa |
| 翻译后修饰 | Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. The N-terminus is blocked. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66673 anti-Podoplanin antibody [SQab19158] IHC-P image
Immunohistochemistry: Paraffin-embedded Human diffuse large B-cell lymphoma tissue stained with ARG66673 anti-Podoplanin antibody [SQab19158].
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ARG66673 anti-Podoplanin antibody [SQab19158] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human lung tissue stained with ARG66673 anti-Podoplanin antibody [SQab19158]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
