ARG54138
anti-Prominin 1 antibody
anti-Prominin 1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Death antibody; Gene Regulation antibody
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Prominin 1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2a |
| 靶点名称 | Prominin 1 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human Prominin-1 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | CORD12; CD133; RP41; Antigen AC133; MCDR2; CD antigen CD133; PROML1; AC133; Prominin-like protein 1; Prominin-1; MSTP061; STGD4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide, 0.1mg/ml BSA and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.1mg/ml BSA, 50% Glycerol |
| 浓度 | 0.4 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PROM1 |
| 背景介绍 | Binds cholesterol in cholesterol-containing plasma membrane microdomains.Proposed to play a role in apical plasma membrane organization of epithelial cells.During early retinal development acts as a key regulator of disk morphogenesis.Involved in regulation of MAPK and Akt signaling pathways.In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
| 细胞定位 | Cell projection > cilium > photoreceptor outer segment. Isoform 1: Apical cell membrane; Multi-pass membrane protein By similarity. Cell projection > microvillus membrane; Multi-pass membrane protein. |
| 产品亮点 | Related products: Prominin1 antibodies; Anti-Mouse IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients |
| 研究领域 | Cancer antibody; Cell Death antibody; Gene Regulation antibody |
| 预测分子量 | 97 kDa |
| 翻译后修饰 | Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. |
检测图片 (2) Click the Picture to Zoom In
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ARG54138 anti-Prominin 1 antibody WB image
Western blot: 30 µg of HUVEC cell lysate stained with ARG54138 anti-Prominin 1 antibody at 1:1000 dilution.
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ARG54138 anti-Prominin 1 antibody WB image
Western blot: 30 µg of HCT116 cell lysate stained with ARG54138 anti-Prominin 1 antibody at 1:1000 dilution.
客户反馈
Good
anti-Prominin 1 antibody
Application：WB
Sample：HCT116
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-Prominin 1 antibody
Application：WB
Sample：HUVEC
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
The protoapigenone analog WYC0209 targets CD133+ cells: A potential adjuvant agent against cancer stem cells in urothelial cancer therapy.
IHC-P / Human
