ARG45178
anti-RAD17 antibody
anti-RAD17 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RAD17 |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RAD17 |
| 抗原物种 | Mouse |
| 抗原 | Recombinant protein containing to mouse RAD17. |
| 偶联标记 | Un-conjugated |
| 別名 | RAD17; RAD17 Checkpoint Clamp Loader Component ; RAD17Sp; Rad24; CCYC; Cell Cycle Checkpoint Protein RAD17; R24L; Cell Cycle Checkpoint Protein (RAD17); RAD17 Homolog (S. Pombe); RF-C Activator 1 Homolog; RF-C/Activator 1 Homolog; RAD1 (S. Pombe) Homolog; Rad17-Like Protein; RAD17 Homolog; RAD1 Homolog; HRAD17; HRad17 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 77 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | RAD17 |
|---|---|
| 全名 | RAD17 Checkpoint Clamp Loader Component |
| 背景介绍 | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013] |
| 生物功能 | Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination. [UniProt] |
| 细胞定位 | Cell membrane. [UniProt] |
| 预测分子量 | 77 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4. |
检测图片 (5) Click the Picture to Zoom In
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ARG45178 anti-RAD17 antibody IHC-P image
Immunohistochemistry: Rat spleen stained with ARG45178 anti-RAD17 antibody at 1 μg/ml dilution.
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ARG45178 anti-RAD17 antibody WB image
Western blot: Rat heart stained with ARG45178 anti-RAD17 antibody at 0.5 μg/ml dilution.
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ARG45178 anti-RAD17 antibody IHC-P image
Immunohistochemistry: Mouse Peyer's patches stained with ARG45178 anti-RAD17 antibody at 1 μg/ml dilution.
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ARG45178 anti-RAD17 antibody WB image
Western blot: Mouse heart and NIH/3T3 stained with ARG45178 anti-RAD17 antibody at 0.5 μg/ml dilution.
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ARG45178 anti-RAD17 antibody FACS image
Flow Cytometry: HEPA1-6 stained with ARG45178 anti-RAD17 antibody at 1 µg/10^6 cells dilution.
