ARG57715
anti-RAP1A + RAP1B antibody
anti-RAP1A + RAP1B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RAP1A + RAP1B |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RAP1A + RAP1B |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from Human RAP1A. |
| 偶联标记 | Un-conjugated |
| 別名 | Ras-related protein Krev-1; C21KG; SMGP21; Ras-related protein Rap-1A; RAP1; KREV1; KREV-1; G-22K; GTP-binding protein smg p21A; K-REV; RAL1B |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse lung | ||||||
| 实际分子量 | 21 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | RAP1A; RAP1B |
|---|---|
| 全名 | RAP1A, member of RAS oncogene family; RAP1B, member of RAS oncogene family |
| 背景介绍 | This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| 生物功能 | Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions. [UniProt] |
| 细胞定位 | Cell membrane, Lipid-anchor, Cytoplasm, perinuclear region, Cell junction, Early endosome. [UniProt] |
| 预测分子量 | RAP1A and RAP1B: 21 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG57715 anti-RAP1A + RAP1B antibody WB image
Western blot: 25 µg of Mouse lung lysate stained with ARG57715 anti-RAP1A + RAP1B antibody at 1:1000 dilution.
