ARG54881
anti-RIPK3 / RIP3 antibody
anti-RIPK3 / RIP3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Signaling Transduction antibody
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概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RIPK3 / RIP3 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RIPK3 / RIP3 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 489-518 of Human RIPK3 / RIP3. |
| 偶联标记 | Un-conjugated |
| 別名 | Receptor-interacting serine/threonine-protein kinase 3; Receptor-interacting protein 3; RIP-3; RIP3; RIP-like protein kinase 3; EC 2.7.11.1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | K562 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9QZL0 Mouse Receptor-interacting serine/threonine-protein kinase 3 Swiss-port # Q9Y572 Human Receptor-interacting serine/threonine-protein kinase 3 |
|---|---|
| 基因名称 | RIPK3 |
| 全名 | receptor-interacting serine-threonine kinase 3 |
| 背景介绍 | The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008] |
| 生物功能 | Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Cell membrane Mitochondrion |
| 产品亮点 | Related products: RIPK3 antibodies; RIPK3 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: RIP1 activation and pathogenesis of NASH Ripoptosome & Necrosome antibody panels are launched |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 57 kDa |
| 翻译后修饰 | RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-199 plays a role in the necroptotic function of RIPK3. Phosphorylation at Ser-227 is required for binding MLKL. Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. |
检测图片 (2) Click the Picture to Zoom In
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ARG54881 anti-RIPK3 / RIP3 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG54881 anti-RIPK3 / RIP3 antibody.
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ARG54881 anti-RIPK3 / RIP3 antibody WB image
Western blot: 35 µg of K562 cell lysate stained with ARG54881 anti-RIPK3 / RIP3 antibody.
客户反馈
Good
anti-RIPK3 / RIP3 antibody
Application：WB
Sample：MCF7
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
