ARG43131
anti-RNF169 antibody
anti-RNF169 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RNF169 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RNF169 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to N311-K708 of Human RNF169. |
| 偶联标记 | Un-conjugated |
| 別名 | E3 ubiquitin-protein ligase RNF169; EC 6.3.2.-; RING finger protein 169 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | ~ 90 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunogen affinity purified. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # E9Q7F2 Mouse E3 ubiquitin-protein ligase RNF169 Swiss-port # Q8NCN4 Human E3 ubiquitin-protein ligase RNF169 |
|---|---|
| 基因名称 | RNF169 |
| 全名 | ring finger protein 169 |
| 生物功能 | Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair. [UniProt] |
| 细胞定位 | Nucleus, nucleoplasm. Note=Localizes to sites of double-strand breaks (DSBs) following DNA damage. Recruited to DSBs via recognition of RNF168-dependent ubiquitin products. [UniProt] |
| 预测分子量 | 77 kDa |
检测图片 (1) Click the Picture to Zoom In
-
ARG43131 anti-RNF169 antibody WB image
Western blot: 50 µg of sample under reducing conditions. U2OS, Caco-2, K562, A431, HEK293, A549 and HeLa whole cell lysates stained with ARG43131 anti-RNF169 antibody at 0.5 µg/ml dilution, overnight at 4°C.
